The Hardin Lab

Time 2020-10-14 10:03:29

Web Name: The Hardin Lab

WebSite: http://worms.zoology.wisc.edu

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UNIVERSITY of WISCONSIN UW INTEGRATIVE BIOLOGY | COLLEGE of LETTERS & SCIENCE THE HARDIN LAB Images in the banner (from left to right and top to bottom): DLG-1/Discs large::GFP during dorsal intercalation [M. K ppen]; Pdlg-1::GFP during ventral enclosure [M. Sheffield]; AJM-1 (green) and muscle (red) during elongation [P. Heid]; HMP-2/beta-catenin electrostatic surface [H.-J. Choi]; AJM-1 (green) and muscle (red) in an elongated embryo [A. Cox-Paulson]; phalloidin staining in elongated embryo [M. Costa]Welcome to the Hardin LabWe use the C. elegans embryo as a model for investigating cell movement and cell adhesion during embryonic development. Understanding how cells move, and how they make and break adhesions has important implications for understanding birth defects during human development and for understanding cancer progression. To find out more, click here...Postdoctoral positions available! Click here for more information Our work is supported by the National Institute of General Medical Sciences, NIH CONTACTJeff Hardin Department of Integrative Biology, University of Wisconsin327 Zoology Research Building 1117 W. Johnson St. Madison, WI 53706voice: (608) 262-9634 fax: (608) 262-7319 lab: (608) 265-2520 email: jdhardin@wisc.eduGRADUATE PROGRAMSUW Integrative Biology  |  Biophysics  |  Cellular & Molecular Biology  |  Genetics  |  Molecular& Cellular PharmacologyRecent NewsAugust 2020The movies on this site have finally been upgraded to the modern HTML5 era! Now they can all be viewed on smart phones and tablets without QuickTime.The cover design for World of the Cell 10th edition has been released! We're making the final push for an early 2021 release date July 2020SRGP-1/srGAP stabilizes adherens junctions in the C. elegans embryo (in preparation).HMP-1 VH2::GFP (green) in a comma stage embryo (Bethany Lucas and Xiangqiang Shao).Hardin, J. and Weliky, M. (2019). Cell rearrangement induced by filopodial tension accounts for the late phase of convergent extension in the sea urchin archenteron. Mol. Biol. Cell 30:1911-1919. PubMed Colorized SEMs of L. pictus gastrulae (J. Hardin).Shao, X., Lucas, B.G., Strauch, J. and Hardin, J. (2019). The adhesion modulation domain of C. elegans -catenin regulates actin binding during morphogenesis. Mol. Biol. Cell 30:2115-2123. PubMed anti-AJM-1 (green) and HMP-1 (red) staining in a hmp-1(jc48) (a CRISPR null allele; Xiangqiang Shao).November 2017Tim's paper got the cover of MBoC for December 2017!Loveless, T., Qadota, H., Benian, GM., and Hardin, J. (2017). C. elegans SORB-1 localizes to integrin adhesion sites and is required for organization of sarcomeres and mitochondria in myocytes. Mol. Bio. Cell, 28:3621-3633. PubMed October 2017srGAP review: Lucas, B. and Hardin, J. (2017). Mind the (sr)GAP roles of Slit-Robo GAPs in neurons, brains and beyond. J. Cell Sci. 130: 3965-3974. PubMed August 2017Fine-mapping -/ -catenin binding interface in vitro and in vivo (with Hee-Jung Choi's group). Shao, X. Kang, H., Loveless, T., Lee, G.R., , Seok, C., Weis, W.I., and Choi, H.-J., and Hardin, J. (2017). Cell cell adhesion in metazoans relies on evolutionarily conserved features of the -catenin -catenin binding interface. J. Biol. Chem. 292,16477 16490. PubMed Surface rendering of HMP-1/ -catenin (green) and HMP-2/ -catenin (aqua). Rendering by Tim LovelessJune 2017Student news: Congratulations to new Genetics PhDs Drs. Bethany Lucas and Xiangqiang Shao, who both defended their theses.May 2017The structure of a C. elegans -catenin. Kang et al (2017). Structural and functional characterization of Caenorhabditis elegans -catenin reveals constitutive binding to -catenin and actin. (with Hee-Jung Choi's group; J. Biol. Chem. 29, 7077-7086). PubMed The N terminus (purple, yellow), M domain (green, aqua) and a homology model of the actin-binding domain (red) of HMP-1/ -catenin fitted into the SAXS envelope of HMP-1. Rendering by Hee-Jung Choi.November 2016Walck-Shannon et al. (2016). CDC-42 orients cell migration during epithelial intercalation in the Caenorhabditis elegans epidermis. PLOS Genetics 12(11): e1006415 PubMed Confocal images of an epidermal cytoplasmic reporter (Plbp-1::GFP) in wild-type (WT) (left) and ZF1::cdc-42; cdc-42(gk388) (right) embryos, in which there is late maternal loss of CDC-42, 45 min. after terminal division. Scale bar is 5 m. [Elise Walck-Shannon] Older NewsFebruary 2016F1000 review of the cadherin/catenin complex in C. elegans. F1000Research 2015, 4(F1000 Faculty Rev):1473 (doi: 10.12688/f1000research.6866.1) PubMed World of the Cell 9th edition (Pearson) is out! Thanks to Marisa Otegui for the beautiful image used on the cover.October 2015Proteomic analysis of the cadherin/catenin complex in C. elegans (collaboration with Jon Audhys's group):Callaci, S., Morrison, K., Shao, X., Schuh, A.L. Wang, Y., Yates III, J.R., Hardin, J., and Audhya, A. (2015). Phosphoregulation of the C. elegans cadherin-catenin complex. Biochemistry 472:339-52.. PubMed hmp-1 mutant embryo rescued with a quadruple mutant phosphomimetic form of HMP-1 tagged with gfp (Xiangqiang Shao).August 2015Dorsal intercalation in C. elegans uses a conserved Trio/CARMIL system upstream of Rac and RhoG. Development 142, 3549-3560. PubMed crml-1(gm326) dorsal cells have excessive protrusive activity, which can be suppressed by GEF1 loss of function n unc-73(rh40) mutants. Left-hand cells are psuedocolored green. Yellow arrows denote excessive, lateral protrusions. Scale bar is 5 m. [Elise Walck-Shannon]July 2015Student news: Congratulations to Elise Walck-Shannon, who defended her thesis July 29, 2015!Blake Martin received a Gilliam fellowship from HHMI!March 2015Angstroms to embryos: structure-function analysis of the cadherin complex in C. elegans(collaboration with Bill Weis, Stanford, and Hee-Jung Choi, Seoul Nat. Univ.; Developmental Cell 33, 82 93) Electrostatic representation of zebrafish -catenin (left) and C. elegans HMP-2 (right). [Hee-Jung Choi, Bill Weis, Tim Loveless]June 2014Minor updates to ImageJ pluginsare available. Go to the Microscopy page.January 2014Cell rearrangement reviewWalck-Shannon, E. and Hardin, J. (2014). Cell intercalation from top to bottom. Nature Rev. Mol. Cell. Bio 15:34-48. Pubmed February 2013Mapping functionally important domains in the C terminus of -cateninMaiden, S.L., Harrison, N., Keegan, J., Cain, B., Lynch, A.M., Pettitt, J., and Hardin, J. Specific conserved C-terminal amino acids of Caenorhabditis elegans HMP-1/ -catenin modulate F-actin binding independently of vinculin. J. Biol. Chem. 288:5694-706. PubMed Homology model of the C terminus o HMP-1/ -catenin, using the metavinculin VH3 domain crystal structure as a template. Residues mutated in hmp-1 alleles are shown as space-filled molecules labeled with the amino acid number. (A) and (B) Residues mutated in strong loss-of-function alleles. (C) Residues mutated in hmp-1(fe4) and intragenic suppressor alleles. [Stephanie Maiden]August 2012MAGI-1, AFD-1/afadin, and the cadherin interactome during morphogenesisLynch, A.M., Grana, T., Cox-Paulson, E., Couthier, A., Cameron, M., Chin-Sang, I., Pettitt, J., and Hardin, J. (2012). A genome-wide functional screen identifies MAGI-1 as an L1CAM-dependent stabilizer of apical junctions in C. elegans. Curr. Biol 22, 1891 1899. PubMed Top: Wild-type embryo expressing an actin reporter during ventral enclosure. Bottom: a magi-1(RNAi) embryo. Cells migrate ventrally, but migration is irregular and cells display excess protrusive activity at the ventral midline. [Allison Lynch]June 2012Tropomodulin protects adherens junctions under stress during morphogenesisCox-Paulson, E., Walck-Shannon, E., Lynch, A., Yamashiro, S., Zaidel-Bar, R., Celeste C. Eno, C., Ono, S., and Hardin, J. (2012). Tropomodulin protects -catenin-dependent junctional actin networks under stress during epithelial morphogenesis. Curr. Biol. 22:1500-1505. PubMed hmp-1/ -catenin and unc-94/tropomodulin synergistically regulate embryonic morphogenesis. Purple, F-actin (phalloidin staining); green, JAC-1/p120ctn::GFP. Left: a hmp-1(fe4) embryo at the 1.5-fold stage. Junctions are largely normal. Right: a hmp-1(fe4);unc-94(RNAi) embryo. Junctions between seam cells and ventral and dorsal epidermal cells have ripped apart [Abbi Cox-Paulson]January 2012Cell migration during ventral enclosureIkegami, R., Simokat, K., Zheng, H., Dixon, L., Garriga, G., Hardin, J. and Culotti, J. (2012). Semaphorin and Eph receptor signaling guide a series of cell movements for ventral enclosure in C. elegans. Curr. Biol. 22:1 11. PubMed Ventral view of the pocket region of an embryo expressing Pplx-2::gfp; anterior at top right. The ventral midline (dashed line) and relative positions of expressing identified P cells (circles, closed circles are P9/10) and plexin expressing cells on the surface of the open ventral pocket (colored squares) are indicated. [Kristin Simokat and Richard Ikegami]November 2011 -catenin reviewMaiden, S.L. and Hardin, J. (2011). The secret life of -catenin: moonlighting during morphogenesis. J. Cell Biol. 195:543 552. PubMed January 2011Loss of the RhoGAP SRGP-1 promotes the clearance of dead and injured cellsNeukomm, L.J., Frei, A.P., Cabello, J., Kinchen, J.M., Zaidel-Bar, R., Ma, Z., Haney, L.B., Hardin, J., Ravichandran, K.S., Moreno, S., and Hengartner, M.O. (2011). Loss of the RhoGAP SRGP1 promotes the clearance of dead and injured cells in Caenorhabditis elegans. Nature Cell Biol. 13:79-86. PubMed ced-6 larvae accumulate cell corpses due to engulfment defects (arrowheads, left). Engulfment defects are suppressed in ced-6;srgp-1 double mutants (right). [Lukas Neukomm]November 2010SRGP-1/srGAP regulates membrane protrusion during cell-cell adhesion(Zaidel-Bar, R., Joyce, M.J., Lynch, A.M., Witte, K., Audhya, A., and Hardin, J. (2010). The F-BAR domain of SRGP-1 facilitates cell-cell adhesion during C. elegans morphogenesis. J. Cell Biol. 191, 761-9.) PubMed JCB In This Issue November 15, 2010 cover!Embryo expressing SRGP-1::GFP (green) stained for actin (purple).The inset shows extensive induced tubulations. [Ronen Zaidel-Bar]August 2010Intramolecular regulation of alpha-catenin's ability to bind actin(Kwiatkowski, A.V., Maiden, S.L., Pokutta, S., Choi, H.-J., Benjamin, J.M., Lynch, A.M., Nelson, W.J., Weis, W.I., and Hardin, J. (2010). In vitro and in vivo reconstitution of the cadherin-catenin-actin complex from Caenorhabditis elegans. PNAS 107,14591-14596.)PubMed August 17, 2010 cover!Color adjusted image of a hmp-1(zu278) mutant embryo stained with phalloidin. [Stephanie Maiden].May 2010Cadherins and L1CAMs during cell-cell adhesion and gastrulation(Grana, T.M., Cox, E.A., Lynch, A.M., and Hardin, J. (2010). SAX-7/L1CAM and HMR-1/cadherin function redundantly in blastomere compaction and non-muscle myosin accumulation. Dev. Biol. 344, 731 744.)PubMed Color adjusted image of a devitellinized hmr-1(RNAi);sax-7(eq1) C. elegans embryo. Blastomeres are loosely adherent and cell division orientations are abnormal. [Theresa Grana]. University logo that links to main university website 2020 Board of Regents of the University of Wisconsin System CONTACT US

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